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Schober
lab at SIUe |
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Research cancer cell motility microtubule and actin signaling microtubule plus-end proteins Techniques plasmid-based RNA interference immunofluorescence-live cell correlative microscopy MetaMorph image analysis flow cytometry cell microinjection |
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Proposed role of
EB1 protein in cell motility. We hypothesize EB1
protein is part of signaling pathways that regulate actin dynamics at the
cell edge. An upstream event (signal) initiates protrusion. Pathways
through EB1 on the plus-ends of
microtubules (MT) regulate actin dynamics (lamellipodia and
filopodia) and adhesion site remodeling. Our preliminary studies support a
model where EB1 promotes lamellipodia protrusion, but suppresses filopodia
and adhesion site formation. |
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Key signaling
molecules in lamellipodial and filopodial protrusion. A stimulus initiates accumulation of
membrane patches rich in lipid second messenger and IQGAP. Rho family GTPases are recruited and
activated. Downstream effectors
(IRSp53, mDia1, WAVE2 and PKC) link Rho GTPases to Arp2/3 complex and fascin
activity. Arp2/3 complex and fascin
act proximal to lamellipodia and filopodia protrusion. EB1, on microtubule plus-ends, localizes to
areas of actin signaling. |
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Hypothesized model of EB1 interaction in cell
motility signaling. Our studies support a model in which
EB1 balances lamellipodia with filopodia modes of protrusion through a pathway
involving PKC and Rac1. |
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SIUe School of Pharmacy Pharmaceutical Sciences Doctor of Pharmacy Degree Biology Research Schober lab
Flickr |
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