Reverse the direction and set the flow rate to be 0.5mL/min (KW-800 series and LW-803) or 0.15mL/min (KW400) series). <p>
The volume of the cleaning solvent required is 5 to 10 times the column volume. ,p>
1) In case where protein has been adsorbed: <p>
Use a solvent containing 0.5M salt or phosphate buffer (about pH3.0).<p><p>
2) In case where a hydrophobic substance has been adsorbed :<p>
Use a solvent containing 10 to 20% acetonitrile or methanol.<p>