B.S., University of Illinois at Springfield, 1994
Ph.D., Pharmacology, University of Illinois at Chicago (UIC), 2003
American Heart Association Postdoctoral Fellow, Northwestern University
Dr. Joseph Schober is an associate professor in the Department of Pharmaceutical Sciences. After receiving a B.S. degree from the University of Illinois at Springfield, he was a practicing Clinical Laboratory Scientist from 1994 to 1996. In 2003, he received a Ph.D. from the Department of Pharmacology, University of Illinois at Chicago (UIC). His research at UIC focused on the interaction of monocyte integrin receptors with novel extracellular matrix proteins expressed in atherosclerotic lesions. From 2003 to 2007, Dr. Schober was a Drug Discovery Scholar and American Heart Association postdoctoral fellow at Northwestern University, Department of Cell and Molecular Biology, where his research focused on investigation of cytoskeleton dynamics and cell motility. His current research at SIUE focuses on cancer cell biology and biomedical engineering approaches to cancer diagnosis and treatment.
Mao Y, Yin Z, Schober JM. Iteratively Training Classifiers for Circulating Tumor Cell Detection. 2015 IEEE Biomedical Engineering Proceedings, 2015 April
Sahar Foroutannejad, Nathan Rohner, Michael Reimer, Guim Kwon, Joseph M Schober. A novel role for IQGAP1 protein in cell motility through cell retraction. Biochemical and Biophysical Research Communications, 2014; 23 39-44.
J. M. Schober, Guim Kwon, Debbie Jayne, Jeanine M. Cain. The microtubule-associated protein EB1 maintains cell polarity through activation of protein kinase C. Biochemical and Biophysical Research Communications, 2012; 417; 67-72.
Ho-Sup Lee, Yulia A. Komarova, Elena S. Nadezhdina, Rana Anjum, John G. Peloquin, J. M. Schober, Oana Danciu, Jeffrey van Haren, Niels Galjart, Steven Gygi, Anna Akhmanova and Gary G. Borisy. Phosphorylation Controls Autoinhibition of CLIP-170. Molecular Biology of the Cell, 2010; 21:2661-2673.
J.M. Schober, Jeanine M. Cain, Yulia A. Komarova and Gary G. Borisy. Migration and actin protrusion in melanoma cells are regulated by EB1 protein. Cancer Letters, 2009:284:30-36.
Recent research has focused on IQGAP1 role in cell motility, and interdisciplinary projects in cancer biomedical engineering. The project with IQGAP1 was founded on the serendipitous discover of its localization in retracting cell edges. We pursued this discovery and are currently working on identifying the domains responsible for localization of IQGAP1 to retracting cell edges. Over the last few years we have developed collaborative biomedical engineering projects involving capture devices for analysis of circulating tumor cells from blood. So far we have developed an image analysis algorithm for enumeration of cancer cells in whole blood samples. This project, now in beginning stages, will hopefully lead to a microfluidics approach for rapid diagnosis and treatment monitoring in cancer patients.
Correlation of cell edge retraction velocity with IQGAP1. (A) B16F10 cells were cotransfected with GFP-IQGAP1 and mCherry-Arp3, and imaged on laminin for a total time of 146 sec at 13-sec intervals. Select time points show IQGAP1, Arp3 and phase images of an area of cell edge retraction. (B) Frames were analyzed for GFP-IQGAP1 and mCherry-Arp3 intensities, and edge velocity changes in the areas marked by the arrowheads.
“On-chip” phase and epifluorescence microscopy of an isolated MCF-7 breast cancer cell. Magnetic beads with anti-epCAM antibodies were mixed for 30 minutes with samples containing pre-stained MCF-7 breast cancer cells. Image shows an isolated MCF-7 breast cancer (arrowhead) with 20 beads attached.
Spheroid growth of B16F10 mouse melanoma cells on 1 kP hydrogels. Single cells were added to 1 kP polyacrylamide gels cross-linked with laminin and incubated for 48 hours.